期刊
ACS CHEMICAL NEUROSCIENCE
卷 9, 期 8, 页码 2009-2015出版社
AMER CHEMICAL SOC
DOI: 10.1021/acschemneuro.8b00124
关键词
Astrocytes; glutamate transport; pH; mitochondria; extracellular potassium; mitochondrial potential; oxygen consumption rate; brain energy metabolism
资金
- Swiss National Science Foundation [31003A-135720]
- Swiss National Science Foundation (SNF) [31003A_135720] Funding Source: Swiss National Science Foundation (SNF)
Astrocytes clear glutamate and potassium, both of which are released into the extracellular space during neuronal activity. These processes are intimately linked with energy metabolism. Whereas astrocyte glutamate uptake causes cytosolic and mitochondrial acidification, extracellular potassium induces bicarbonate-dependent cellular alkalinization. This study aimed at quantifying the combined impact of glutamate and extracellular potassium on mitochondrial parameters of primary cultured astrocytes. Glutamate in 3 mM potassium caused a stronger acidification of mitochondria compared to cytosol. 15 mM potassium caused alkalinization that was stronger in the cytosol than in mitochondria. While the combined application of 15 mM potassium and glutamate led to a marked cytosolic alkalinization, pH only marginally increased in mitochondria. Thus, potassium and glutamate effects cannot be arithmetically summed, which also applies to their effects on mitochondrial potential and respiration. The data implies that, because of the nonlinear interaction between the effects of potassium and glutamate, astrocytic energy metabolism will be differentially regulated.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据