期刊
ACS APPLIED MATERIALS & INTERFACES
卷 10, 期 31, 页码 26075-26083出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.8b09152
关键词
DNA hydrogel; silver clusters; multicolor cell imaging; reactive oxygen/nitrogen species; ratiometric fluorescent probe
资金
- National Natural Science Foundation of China [51503096, 21705074]
- Shenzhen Fundamental Research Programs [JCYJ20160226193029593]
- Guangdong Innovative and Entrepreneurial Research Team Program [2016ZT06G587]
Oligonucleotide-stabilized silver nanoclusters (AgNCs) show promising applications in bioimaging and bio-/chemo-sensing. However, their unsatisfactory photostability limits their practical applications. In this work, fluorescent AgNCs were synthesized in situ in a DNA hydrogel, consisting of cross-linked enzymatically amplified polymeric DNAs with cytosine-rich sequences in the presence of Agt The fluorescence property of the resultant AgNCs was optimized by a rational design of the DNA sequences to cover a broad spectrum with comparable green and red emissions. Under the protection of the DNA hydrogel, the AgNCs showed significantly improved photostability in an ambient oxygen environment, as well as low cytotoxicity even at a high concentration. Therefore, these properties show the rolling-circle-amplification stabilized AgNCs to be a promising possible fluorescent probe for the detection of reactive oxygen/nitrogen species (ROS/RNS) in live cells because red-emitting species are susceptible to oxidation and consequently convert to green-emitting species. Finally, the as-prepared AgNCs were demonstrated to be a sensitive and specific probe for cellular imaging and the monitoring of ROS/RNS levels, which broadens the applications of AgNCs and provides a new tool for related biological investigations.
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