期刊
ACS APPLIED MATERIALS & INTERFACES
卷 10, 期 15, 页码 12364-12373出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.7b18592
关键词
self-assembly; surface-enhanced Raman scattering; statistical spectral analysis; biofilms; metabolomics; quorum sensing; biosensor
资金
- National Science Foundation [EECS-1449397]
- National Science Foundation BEST IGERT Program [NSF DGE-1144901]
- Schlumberger Foundation
- AFOSR [FA9550-17-1-0913]
- Directorate For Engineering [1449397] Funding Source: National Science Foundation
- Div Of Electrical, Commun & Cyber Sys [1449397] Funding Source: National Science Foundation
Detection of bacterial metabolites at low concentrations in fluids with complex background allows for applications ranging from detecting biomarkers of respiratory infections to identifying contaminated medical instruments. Surface-enhanced Raman scattering (SERS) spectroscopy, when utilizing plasmonic nanogaps, has the relatively unique capacity to reach trace molecular detection limits in a label-free format, yet large-area device fabrication incorporating nano gaps with this level of performance has proven difficult. Here, we demonstrate the advantages of using chemical assembly to fabricate SERS surfaces with controlled nanometer gap spacings between plasmonic nanospheres. Control of nanogap spacings via the length of the chemical crosslinker provides uniform SERS signals, exhibiting detection of pyocyanin, a secondary metabolite of Pseudomonas aeruginosa, in aqueous media at concentration of 100 pg center dot mL(-1). When using machine learning algorithms to analyze the SERS data of the conditioned medium from a bacterial culture, having a more complex background, we achieve 1 ng center dot mlL(-1) limit of detection of pyocyanin and robust quantification of concentration spanning 5 orders of magnitude. Nanogaps are also incorporated in an in-line microfluidic device, enabling longitudinal monitoring of P. aeruginosa biofilm formation via rapid pyocyanin detection in a medium effluent as early as 3 h after inoculation and quantification in under 9 h. Surface-attached bacteria exposed to a bactericidal antibiotic were differentially less susceptible after 10 h of growth, indicating that these devices may be useful for early intervention of bacterial infections.
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