期刊
ACS APPLIED MATERIALS & INTERFACES
卷 10, 期 5, 页码 4494-4501出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.7b17653
关键词
antibiotic resistance genes; paper-based chip; loop-mediated isothermal amplification; [Ru(phen)(2)dppz](2+); multiple detection
资金
- National Natural Science Foundation of China [21475048]
- National Science Fund for Distinguished Young Scholars of Guangdong Province [2014A030306008]
- Project of Guangzhou Science and Technology Plan [201508020003]
- Program of the Pearl River Young Talents of Science and Technology in Guangzhou [2013J2200021]
- Special Support Program of Guangdong Province [2014TQ01R599]
- Outstanding Young Teacher Training Program of Guangdong Province [HS2015004]
Antibiotic resistance, the ability of some bacteria to resist antibiotic drugs, has been a major global health burden due to the extensive use of antibiotic agents. Antibiotic resistance is encoded via particular genes; hence the specific detection of these genes is necessary for diagnosis and treatment of antibiotic resistant cases. Conventional methods for monitoring antibiotic resistance genes require the sample to be transported to a central laboratory for tedious and sophisticated tests, which is grueling and time-consuming. We developed a paper-based chip, integrated with loop-mediated isothermal amplification (LAMP) and the light switch molecule [Ru(phen)(2)dppz](2+), to conduct turn-on fluorescent detection of antibiotic resistance genes. In this assay, the amplification reagents can be embedded into test spots of the chip in advance, thus simplifying the detection procedure. [Ru(phen)(2)dppz](2)(+) was applied to intercalate into amplicons for product analysis, enabling this assay to be operated in a wash-free format. The paper-based detection device exhibited a limit of detection (LOD) as few as 100 copies for antibiotic resistance genes. Meanwhile, it could detect antibiotic resistance genes from various bacteria. Noticeably, the approach can be applied to other genes besides antibiotic resistance genes by simply changing the LAMP primers. Therefore, this paper-based chip has the potential for point-of-care (POC) applications to detect various gene samples, especially in resource-limited conditions.
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