期刊
ACS APPLIED MATERIALS & INTERFACES
卷 10, 期 10, 页码 9060-9068出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.8b00327
关键词
dual-template epitope imprinting; molecular imprinting polymer; thermosensitive recognition; metal chelation imprinting; distillation-precipitation polymerization
资金
- National Natural Science Foundation of China [21275078, 21475069, 21775077]
- Tianjin Natural Science Foundation [16JCZDJC37200]
A new type of thermosensitive dual-template epitope molecular imprinting polymer was prepared and coated on magnetic carbon nanotubes (MCNTs@D-EMIP) for simultaneous recognition of human serum albumin (HSA) and transferrin (Trf) via the strategies of dual-template epitope imprinting, metal chelation imprinting, and distillation precipitation polymerization (DPP). C-terminal peptides of HSA and C-terminal peptides of Trf were selected as templates, zinc acrylate and N-isopropylacrylamide were used as functional monomers, and MCNTs@D-EMIP was prepared by the method of DPP. The two types of template epitopes were immobilized by metal chelation and six-membered ring formed with zinc acylate. MCNTs@D-EMIP was prepared in only 30 min, which was much shorter than other polymerization methods. The resultant MCNTs@D-EMIP showed excellent specific recognition ability toward HSA and Trf. The adsorption amounts of MCNTs@D-EMIP for HSA and Trf were 103.67 and 68.48 mg(-1) and the imprinting factors were 2.57 and 2.17, respectively. In addition, MCNTs@D-EMIP displayed a thermosensitive property to realize temperature controlled recognition and release of target proteins. Furthermore, the results of high-performance liquid chromatography analysis proved that MCNTs@D-EMIP could be applied to specifically recognize two types of targets simultaneously in the biosample. The proposed strategy provided a preparation method for the thermosensitive dual-template epitope imprinting polymer via dual-template imprinting, metal chelation imprinting, and DPP.
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