期刊
ACS APPLIED MATERIALS & INTERFACES
卷 10, 期 17, 页码 14483-14490出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.8b02262
关键词
electrochemiluminescence; double-output amplification; MoS2 nanoflowers; co-reaction accelerator; aptasensor
资金
- NNSF of China [21675130, 21775124, 21675129, 21575116]
- Fundamental Research Funds for the Central Universities, China [XDJK2018AA003, XDJK2015A002]
In this work, a sensitive electrochemiluminescent assay of mucin 1 (MUC1) was developed with the advantages of target recycling amplification strategy and effective MoS2 nanoflower (MoS2 NF)-based signal probe. Briefly, the target MUC I triggered enzyme-free recycling and a double-output amplification process was executed to acquire masses of single-stranded DNA as a mimic target, which further participated in the catalytic hairpin assembly process for signal amplification. Meanwhile, MoS2 NFs were prepared as an effective co-reaction accelerator, which not only possessed excellent catalytic performance for H2O2 decomposition to largely enhance the luminous intensity of N-(aminobutyl)-N-(ethylisoluminol) (ABEI)-H2O2 electrochemiluminescence system but also offered a desirable platform for ABEI-functionalized Ag nanoparticles (ABEI-Ag complexes) loading via Ag-S binding. The experimental results showed the proposed aptasensor had a good linear relationship in the range of 1 fg/mL to 10 ng/mL for MUC1 detection and the limit of detection was 0.58 fg/mL (S/N = 3). In addition, the aptasensor had nice stability and selectivity and huge potential to be applied in clinical research.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据