期刊
ACS APPLIED MATERIALS & INTERFACES
卷 10, 期 17, 页码 14410-14417出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.8b00952
关键词
aggregation-induced emission; calcium ions; light-up detection; hypercalcemia; bone microcracks
资金
- National Natural Science Foundation of China [21788102, 51620105009, 21602063]
- National Key R&D Program of China [2017YFC1105004, 2017YFC1103402]
- Science and Technology Planning Project of Guangzhou [201607020015, 201704030069]
- Pearl River S&T Nova Program of Guangzhou [201806010152]
- Natural Science Foundation of Guangdong Province [2016A030313852, 2016A030312002]
- Fundamental Research Funds for the Central Universities [2015ZY013, 2015ZZ104]
- Innovation and Technology Commission of Hong Kong [ITC-CNERC14SC01]
- Guangdong Innovative Research Team Program [201101C0105067115]
The fluorescent probe for the detection of calcium ions is an indispensable tool in the biomedical field. The millimolar order of Ca(II) ions is associated with many physiological processes and diseases, such as hypercalcemia, soft tissue calcification, and bone microcracks. However, the conventional fluorescent probes are only suitable for imaging Ca(II) ions in the nanomolar to micromolar range, which can be because of their high affinities toward Ca(II) ions and aggregation-caused quenching drawbacks. To tackle this challenge, we herein develop an aggregation-induced emission (ME) probe SA-4CO(2)Na for selective and light-up detection of Ca(II) ions in the millimolar range (0.6-3.0 mM), which can efficiently distinguish between hypercalcemic (1.4-3.0 mM) and normal (1.0-1.4 mM) Ca2+ ion levels. The formation of fibrillar aggregates between SA-4CO(2)Na and Ca(II) ions was clearly verified by fluorescence, scanning electron microscopy, and transmission electron analysis. Moreover, this AIE-active probe can be used for wash-free and light-up imaging of a high concentration of Ca(II) ions even in the solid analytes, including calcium deposits in psammomatous meningioma slice, microcracks on bovine bone surface, and microdefects on hydroxyapatite-based scaffold. It is thus expected that this ME-active probe would have broad biomedical applications through light-up imaging and sensing of Ca(II) ions at the millimolar level.
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