期刊
ACS APPLIED MATERIALS & INTERFACES
卷 10, 期 17, 页码 14399-14409出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.8b00802
关键词
hydrogel interfaces; thiol disulfide exchange chemistry; bioconjugation; reversible conjugation; protein and cell attachment
资金
- Ministry of Development of Turkey [2009K120520, 2012K120480]
Surfaces engineered to specifically capture and release on demand analytes ranging from biomolecules to cells find niche applications in areas such as diagnostics and detection. Utilization of a disulfide-based linker as a building block allows fabrication of a novel hydrogel-based platform that incorporates a catch and release attribute. Hydrogels incorporating pyridyl disulfide groups as thiol-reactive handles were prepared by photopolymerization in the presence of a poly(ethylene glycol) (PEG)-based cross-linker. A range of bulk and micropatterned hydrogels with varying amounts of the reactive group were prepared using PEG -based monomers with different chain lengths. Thiol-containing molecules were conjugated to these hydrogels through the thiol disulfide exchange reaction under ambient conditions with high efficiencies, as determined by UV-vis spectroscopy. Facile conjugation of a thiol-containing fluorescent dye, namely 4,4-difluoro-1,3,5,7-tetramethy1-8-[(10-mercapto)]-4-bora-3a,4a-diaza-s-indacene, was demonstrated, followed by its effective cleavage in the presence of dithiothreitol (DTT), a thiol-containing disulfide-reducing agent. Conjugation of a biotin-containing ligand onto the hydrogels allowed specific binding of protein extravidin when exposed to a mixture of extravidin and bovine serum albumin. The bound protein could be released from the hydrogel by simple exposure to a DTT solution. Likewise, hydrogels modified with a cell-adhesive peptide unit containing the RGD sequence acted as favorable substrates for cellular attachment. Incubation of these cell-attached hydrogel surfaces in a DTT-containing solution leads to facile detachment of cells from the surfaces, while retaining a high level of cell viability. It can be envisioned that the benign nature of these hydrogels, their facile fabrication, and modular functionalization will make them attractive platforms for many applications.
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