期刊
ACS APPLIED MATERIALS & INTERFACES
卷 10, 期 8, 页码 6942-6955出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.7b18896
关键词
mitochondria targeting; nanographene; NIR photosensitizer; CpG oligonucleotides; combination therapy
资金
- National Natural Science Foundation of China [81471785, 81671821, 31470959, 31470906, 11502049, 11772088, 31700811]
- Basic Research Program of Sichuan Science and Technology [2017JY0217, 2017JY0019]
- Fundamental Research Funds for the Central Universities [ZYGX2015J143, ZYGX2016Z001]
Nanomedicine-based combination therapy has sparked a growing interest in clinical disease treatment and pharmaceutical industry. In this study, a mitochondria-targeted and near-infrared (NIR) light-activable multitasking nano-graphene (i.e., GT/IR820/DP-CpG) was engineered to in situ trigger highly efficient triple-punch strategy of cancer photo dynamic therapy, photothermal therapy, and immunotherapy. Modification of triphenylphosphonium on graphene made the vehicle specifically guide the NIR dye IR820 home to mitochondria, followed by lysosomes escape in a time-dependent manner. The photoactive nanocomplex generated an abundant reactive oxygen species as well as photothermal heat to ultimately kill cancer cells by inducing mitochondrial collapse and irreversible cell apoptosis upon the NIR laser irradiation. Further introduction of an immunostimulatory conjugate DP-CpG significantly promoted the secretion of proinflammatory cytokines (i.e., interleukin-6, tumor necrosis factor-alpha, and interferon-gamma) and thus improved the immunogenicity of tumors. In vivo studies demonstrated that GT/IR820/DP-CpG remarkably inhibited tumor growth (tumor inhibition rate, similar to 88%) resulting from the combinational phototherapeutic effect of IR820 and immunostimulatory activity of DP-CpG, thereby causing negligible toxic effects on mice. Our work provides a new paradigm of architecting organelle-targeted and stimulative nanocomplex for highly efficient cancer photoimmunotherapy.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据