期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 17, 期 7, 页码 -出版社
MDPI
DOI: 10.3390/ijms17071031
关键词
Aspergillus nidulans; WDR gene; beta-flanking gene; chitin synthase; collinear genes; cell wall
资金
- Austrian Science Fund (FWF) [M1315]
- Swedish Governmental Agency for Innovation Systems
- VINNOVA
- Swedish Energy Agency, Energimyndigheten
- Lower Austria Science Fund (NFB) [LS12-009]
- Austrian Science Fund (FWF) [M1315] Funding Source: Austrian Science Fund (FWF)
WD40 repeat (WDR) proteins are pleiotropic molecular hubs. We identify a WDR gene that is a conserved genomic neighbor of a chitin synthase gene in Ascomycetes. The WDR gene is unique to fungi and plants, and was called Fungal Plant WD (FPWD). FPWD is within a cell wall metabolism gene cluster in the Ascomycetes (Pezizomycotina) comprising chsD, a Chs activator and a GH17 glucanase. The FPWD, AN1556.2 locus was deleted in Aspergillus nidulans strain SAA.111 by gene replacement and only heterokaryon transformants were obtained. The re-annotation of Aspergilli genomes shows that AN1556.2 consists of two tightly linked separate genes, i.e., the WDR gene and a putative beta-flanking gene of unknown function. The WDR and the beta-flanking genes are conserved genomic neighbors localized within a recently identified metabolic cell wall gene cluster in genomes of Aspergilli. The heterokaryons displayed increased susceptibility to drugs affecting the cell wall, and their phenotypes, observed by optical, confocal, scanning electron and atomic force microscopy, suggest cell wall alterations. Quantitative real-time PCR shows altered expression of some cell wall-related genes. The possible implications on cell wall biosynthesis are discussed.
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