期刊
ACADEMIC RADIOLOGY
卷 26, 期 2, 页码 170-178出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.acra.2018.05.006
关键词
Functional magnetic resonance imaging; Lung transplantation; Fluorinated gas MRI; Oxygen-enhanced imaging; Ex-vivo lung perfusion; Pulmonary ischemic injury evaluated with fMRI
资金
- Federal Ministry of Education and Research via Integrated Research and Treatment Center Transplantation [SU-B06, OPEX-4]
- Hannover Medical School [HiLF 792010049]
- DZL German Center for Lung Research, BREATH
- Fritz-Behrens-Foundation, Hannover, Germany
- DFG
Rationale and Objectives: To evaluate the feasibility of multiparametric magnetic resonance imaging (MRI) of the lungs to detect impaired organ function in a porcine model of ischemic injury within an ex-vivo lung perfusion system (EVLP) prior to transplantation. Materials and Methods: Twelve pigs were anesthetized, and left lungs were clamped to induce warm ischemia for 3 hours. Right lungs remained perfused as controls. Lungs were removed and installed in an EVLP for 12 hours. Lungs in the EVLP were imaged repeatedly using computed tomography, proton MRI (H-1-MRI) and fluorine MRI (F-19-MRI). Dynamic contrast-enhanced derived parenchymal blood volume, oxygen washout times, and F-19 washout times were calculated. P-aO2 was measured for ischemic and normal lungs, wet/dry ratio was determined, histologic samples were assessed, and cytokines in the lung tissue were analyzed. Statistical analysis was performed using nonparametric testing. Results: Eleven pigs were included in the final analysis. Ischemic lungs showed significantly higher wet/dry ratios (p = 0.024), as well as IL-8 tissue levels (p = 0.0098). Histologic assessment as well as morphologic scoring of computed tomography and H-1-MRI did not reveal significant differences between ischemic and control lungs. F-19 washout (p = 0.966) and parenchymal blood flow (p = 0.32) were not significantly different. Oxygen washout was significantly prolonged in ischemic lungs compared to normal control lungs at the beginning (p = 0.018) and further prolonged at the end of the EVLP run (p = 0.005). Conclusion: Muttiparametrtc pulmonary MRI is feasible in lung aflografts within an EVLP system. Oxygen-enhanced imaging seems to be a promising marker for ischemic injury, enabling detection of affected lung segments prior to transplantation.
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