期刊
SLAS DISCOVERY
卷 22, 期 10, 页码 1182-1192出版社
SAGE PUBLICATIONS INC
DOI: 10.1177/2472555217712659
关键词
quantitative MSI; tryptophan; kynurenine; IDOI; biomarker
资金
- la MEL (Metropole Europeenne de Lille), la Region Nord Pas de Calais et BPI France [A1408004N]
- Wallonia Region
Inhibition of NK and effector T-cell functions and activation of regulatory cell populations are the main immunosuppressive effects of indoleamine-2,3-dioxygenaseI (IDOI). By converting tryptophan (Trp) into kynurenine (Kyn), IDOI is involved in the immune response homeostasis, and its dysregulated expression is described in immune-related pathologies, as tumors that hijack it to evade immune destruction. Thereby, IDOI inhibitors are being developed to stimulate antitumor immune responses. Existing and standard quantitation methods of IDOI substrate and metabolite(s) are based on the total level of Trp and its metabolites determined by liquid chromatography tandem mass spectrometry analysis in human plasma, cerebrospinal fluid, and brain. Here, we describe the detection, localization, and absolute quantitation of Trp and Kyn by quantitative mass spectrometry imaging (qMSI) in transfected murine tumor models expressing various levels of IDOI. Myeloid, glycolysis metabolic signatures, and correlation between IDOI expression and Trp to Kyn conversion are also shown. High-definition IDOI and GCN2 immunostainings overlaid with Kyn molecular images underline the tumor metabolism and heterogeneity. The development of immunotherapies such as IDOI inhibitors requires a deep understanding of the immune system, the interplay of cancer cells, and biomarker characterization. Our data underline that qMSI allows the study of the spatial distribution and quantitation of endogenous immune metabolites for biology and pharmacology studies.
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