期刊
BULLETIN OF THE KOREAN CHEMICAL SOCIETY
卷 38, 期 3, 页码 384-388出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/bkcs.11096
关键词
Metalloproteinase-1; Glycan structure; Glycan interaction; beta 1,6-N-acetylglucosamine linkage
资金
- Korea Institute of Science and Technology [2E26110, 2E26990]
- Ministry of Science, Information & Communication Technology and Future Planning
- Ministry of Science, ICT & Future Planning, Republic of Korea [2E26110] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- Ministry of Science & ICT (MSIT), Republic of Korea [2E26990] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Matrix metalloproteinases (MMPs) are proteolytic enzymes that can regulate the tumor microenvironment. Metalloproteinase-1 (TIMP-1) is a MMP inhibitor that plays a critical role in the invasion and migration of cancer cells. N-acetylglucosaminyltransferase-V (GnT-V) catalyzes the attachment of a beta 1,6-Nacetylglucosamine (GlcNAc) linkage to the core glycan, and TIMP-1 has identified target proteins for GnT-V. Recent research reveals that aberrantly glycosylated TIMP-1 showed a weaker inhibition on gelatinase, and that this aberrancy of glycosylation was closely related with cancer cell invasion and metastasis. However, the mechanism of action of glycan modification is not known at the molecular level. In this study, the bi-antennary and aberrant glycan structures were determined by mass spectrometry, and a model of the glycosylated TIMP-1-MMP2 complex was constructed to study the effects of glycosylation on the inhibitory activity of TIMP-1. Then this model was used to examine the effects of attaching a beta 1,6GlcNAc linkage to the core glycan on the interaction of TIMP-1 with MMP2. The gelatinase inhibitory activity is decreased when additional a beta 1,6-GlcNAc moiety is linked to the core glycan on TIMP-1. The modeled structure of the glycosylated TIMP-1-MMP2 complex reveals how aberrant N-linked glycan hinders the interaction of these molecules.
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