期刊
REGENERATIVE THERAPY
卷 6, 期 -, 页码 52-64出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.reth.2016.12.001
关键词
Adipose tissue-derived regenerative cells; Stromal cells; Stem cells; Liver cirrhosis
资金
- Japanese Ministry of Health, Labour and Welfare [25040101]
- Japan Agency for Medical Research and Development [15bk0104002h0003]
- Kanazawa University Hospital
- Grants-in-Aid for Scientific Research [17K09414, 17K09413] Funding Source: KAKEN
Introduction: Adipose tissue stromal cells contain a substantial number of mesenchymal stem cells. As such, their application to regeneration of miscellaneous impaired organs has attracted much attention. Methods: We designed a clinical study to investigate freshly isolated autologous adipose tissue-derived stromal/ stem (regenerative) cell (ADRC) therapy for liver cirrhosis and conducted treatment in four cirrhotic patients. ADRCs were isolated from autologous subcutaneous adipose tissue obtained by the liposuction method, followed with use of the Celution system adipose tissue dissociation device. The primary endpoint is assessment of safety one month after treatment. We also characterized the obtained ADRCs. Results: Two patients had type C cirrhosis, one had nonalcoholic steatohepatitis-cirrhosis, and one had type B cirrhosis. No serious adverse events were observed during the 1-month study period after freshly isolated ADRC infusion. Serum albumin concentrations were maintained or improved during this period as well as during the succeeding follow-up of approximately 1 year in two patients and 6 months in another patient. Liver regeneration-related factors, namely hepatocyte growth factor and interleukin-6, were elevated 1 day after ADRC treatment in all patients. The obtained freshly isolated ADRCs were expanded in culture and found to express mesenchymal stem cell markers. Gene expression profile analysis of ADRCs was shown to involve inflammatory features, suggesting that characteristics of the obtained ADRCs were related to immunomodulatory biological effects. Conclusion: This clinical study treatment for liver cirrhosis using ADRCswas proven to be safely conductible, and can be further investigated in future for regeneration/ repair of liver cirrhosis. (C) 2017, The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据