期刊
FOOD SCIENCE & NUTRITION
卷 6, 期 2, 页码 400-416出版社
WILEY
DOI: 10.1002/fsn3.569
关键词
Citri Reticulatae Pericarpium; cluster analysis; flavonoids; GC-MS; HPLC; volatile oil
资金
- National Natural Science Foundation of China [31401613]
To discriminate the feasible differences and find potential similarities and relationships of Citri Reticulatae Pericarpium (CRP), this work was accomplished by a comprehensive and reliable method using gas chromatography-mass spectrometer (GC-MS) to analyze the volatile oils and high-performance liquid chromatography (HPLC) simultaneously to determine the contents of five bioactive flavonoids, namely hesperidin, nobiletin, 3,5,6,7,8,3,4-heptamethoxyflavone, tangeretin, and 5-hydroxy-6,7,8,3,4-pentamethoxyflavone in 25 batches of CRP samples of 10 cultivars collected from different regions in China. The GC-MS analyses indicated that 98 compounds were successfully identified from the volatile oils obtained and the major constituents of volatile oil are d-limonene, -terpinene, -pinene, linalool, and myrcene. Even 2-(methylamino) benzoate was found in all cultivar samples harvested at maturation stage. Under the optimal condition, the quantitative analyses of five bioactive flavonoids were successfully performed by HPLC and hierarchical cluster analysis (HCA). Results showed significant differences among cultivars in the contents of five bioactive flavonoids mentioned earlier. The HCA and GC-MS results provided a convenient approach which might be applied for rapid similarity evaluation and also holds the potential for analysis of compounds present in other plants. Therefore, this work obtained offers scientific basis to control quality and develop medicinal value of the medicinal materials in CRP.
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