期刊
AMERICAN JOURNAL OF CHINESE MEDICINE
卷 43, 期 3, 页码 457-476出版社
WORLD SCIENTIFIC PUBL CO PTE LTD
DOI: 10.1142/S0192415X15500299
关键词
Apocynum venetum; Flavonoids; Acetaminophen; Hepatotoxicity; Oxidative Stress; HPLC-MS/MS
资金
- High-End Foreign Experts Recruitment Program of State Administration of Foreign Experts Affairs [GDW20146100228]
- State Key Laboratory of Natural Medicines, China Pharmaceutical University [SKLNMKF201221]
- International S&T Cooperation Project of Northwest AF University
- Ministry of Education and State Administration of Foreign Experts Affairs Overseas Teacher Project [MS2011XBNL057]
Apocynum venetum L. (A. venetum) has long been used in oriental folk medicine for the treatment of some liver diseases; however, the underlying mechanisms remain to be fully elucidated. Acetaminphen (APAP) is a widely used analgesic drug that can cause acute liver injury in overdose situations. In this study, we investigated the potential protective effect of A. venetum leaf extract (ALE) against APAP-induced hepatotoxicity. Mice were intragastrically administered with ALE once daily for 3 consecutive days prior to receiving a single intraperitoneal injection of APAP. The APAP group showed severe liver injury characterized by the noticeable fluctuations in the following parameters: serum aminotransferases; hepatic malondialdehyde (MDA), 3-nitrotyrosine (3-NT), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR) and glutathione (GSH). These liver damages induced by APAP were significantly attenuated by ALE pretreatments. A collective analysis of histopathological examination, DNA laddering and western blot for caspase-3 and cytochrome c indicated that the ALE is also capable of preventing APAP-induced hepatocyte death. Hyperoside, isoquercitrin and their derivatives have been identified as the major components of ALE using HPLC-MS/MS. Taken together, the A. venetum possesses hepatoprotective effects partially due to its anti-oxidant action.
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