期刊
MICROBIAL BIOTECHNOLOGY
卷 10, 期 4, 页码 933-957出版社
WILEY
DOI: 10.1111/1751-7915.12731
关键词
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资金
- Ohio State University College of Veterinary Medicine [2013-22]
- National Institute of Allergy and Infectious Diseases [HHSN272200900009C, R01 AI 047885]
- NIH [T35 OD10977]
- American Veterinary Medical Association (AVMA)/American Veterinary Medical Foundation (AVMF) Second Summer Research Opportunity Scholarship
Neorickettsia helminthoeca, a type species of the genus Neorickettsia, is an endosymbiont of digenetic trematodes of veterinary importance. Upon ingestion of salmonid fish parasitized with infected trematodes, canids develop salmon poisoning disease (SPD), an acute febrile illness that is particularly severe and often fatal in dogs without adequate treatment. We determined and analysed the complete genome sequence of N.helminthoeca: a single small circular chromosome of 884232bp encoding 774 potential proteins. N.helminthoeca is unable to synthesize lipopolysaccharides and most amino acids, but is capable of synthesizing vitamins, cofactors, nucleotides and bacterioferritin. N.helminthoeca is, however, distinct from majority of the family Anaplasmataceae to which it belongs, as it encodes nearly all enzymes required for peptidoglycan biosynthesis, suggesting its structural hardiness and inflammatory potential. Using sera from dogs that were experimentally infected by feeding with parasitized fish or naturally infected in southern California, Western blot analysis revealed that among five predicted N.helminthoeca outer membrane proteins, P51 and strain-variable surface antigen were uniformly recognized. Our finding will help understanding pathogenesis, prevalence of N.helminthoeca infection among trematodes, canids and potentially other animals in nature to develop effective SPD diagnostic and preventive measures. Recent progresses in large-scale genome sequencing have been uncovering broad distribution of Neorickettsia spp., the comparative genomics will facilitate understanding of biology and the natural history of these elusive environmental bacteria.
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