期刊
EBIOMEDICINE
卷 25, 期 -, 页码 66-72出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ebiom.2017.10.003
关键词
Malaria; Real-time PCR; msp2-PCR; Gametocyte; Traveler; Treatment
资金
- Swedish Research Council [5212012-3311, 348-2013-6573]
- Stockholm County Council [20130207]
- Swedish Society for Medical Research (SSMF), Stiftelsen Sigurd och Elsa Goljes Minne
- Karolinska Institutet Research Foundation
The rapid clearance of malaria parasite DNA from circulation has widely been accepted as a fact without being systemically investigated. We assessed the persistence of parasite DNA in travelers treated for Plasmodium falciparum malaria in a malaria-free area. Venous blood was collected at the time of admission and prospectively up to one year. DNA and RNA were extracted and analyzed using species-specific and gametocyte-specific real-time PCR as well as merozoite surface protein 2 (msp2)-PCR. In 31 successfully treated individuals, asexual parasites were seen by microscopy until two days after treatment, whereas parasite DNA was detected by msp2- and species-specific PCR up to days 31 and 42, respectively. Statistical modelling predicted 26% (+/- 0.05 SE) species-specific PCR positivity until day 40 and estimated 48 days for all samples to become PCR negative. Gametocytes were detected by microscopy and PCR latest two days after treatment. C-T values correlated well with microscopy-defined parasite densities before but not after treatment started. These results reveal that PCR positivity can persist several weeks after treatment without evidence of viable sexual or asexual parasites, indicating that PCR may overestimate parasite prevalence after treatment. (C) 2017 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据