期刊
EBIOMEDICINE
卷 20, 期 -, 页码 182-192出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ebiom.2017.04.018
关键词
Viral miRNAs; KSHV; HHV8; EBV; HHV4; Infection prevalence
资金
- National Institutes of Health (NIH/NCATS) through the NIH Common Fund, Office of Strategic Coordination (OSC) [UH3TR00943-01]
- NIH/NCI [1 R01 CA182905-01]
- UT MD Anderson Cancer Center SPORE in Melanoma from NCI [P50 CA093459]
- AIM at Melanoma Foundation
- Miriam and Jim Mulva research funds
- UT MD Anderson Cancer Center Brain SPORE [2P50CA127001]
- Developmental Research award from Leukemia SPORE
- CLL Moonshot Flagship
- Knowledge GAP MDACC grant
- Owens Foundation
- Estate of C. G. Johnson
- NCI [P50 CA140388]
- NIH Clinical Research Loan Repayment Program
- CNCSUEFISCDI [22, PN-II-ID-PCE-2012-40018]
- Romanian National Research Council (CNCS) Complex Exploratory Research Projects [CEEX 187/2006]
- UTMD Anderson Cancer Center Institutional Research Grant
- NIH [R01GM116184, R01GM097747, U19AI062629, R21AI113020]
Prevalence of Kaposi sarcoma-associated herpesvirus (KSHV/HHV-8) varies greatly in different populations. We hypothesized that the actual prevalence of KSHV/HHV8 infection in humans is underestimated by the currently available serological tests. We analyzed four independent patient cohorts with post-surgical or post-chemotherapy sepsis, chronic lymphocytic leukemia and post-surgical patients with abdominal surgical interventions. Levels of specific KSHV-encoded miRNAs were measured by reverse transcription-quantitative polymerase chain reaction (RT-qPCR), and KSHV/HHV-8 IgG were measured by immunoassay. We also measured specific miRNAs from Epstein Barr Virus (EBV), a virus closely related to KSHV/HHV-8, and determined the EBV serological status by ELISA for Epstein-Barr nuclear antigen 1 (EBNA-1) IgG. Finally, we identified the viral miRNAs by in situ hybridization (ISH) in bone marrow cells. In training/validation settings using independent multi-institutional cohorts of 300 plasma samples, we identified in 78.50% of the samples detectable expression of at least one of the three tested KSHV-miRNAs by RT-qPCR, while only 27.57% of samples were found to be seropositive for KSHV/HHV-8 IgG (P < 0.001). The prevalence of KSHV infection based on miRNAs qPCR is significantly higher than the prevalence determined by seropositivity, and this ismore obvious for immuno-depressed patients. Plasma viral miRNAs quantification proved that EBV infection is ubiquitous. Measurement of viral miRNAs by qPCR has the potential to become the gold standard method to detect certain viral infections in clinical practice. (C) 2017 Elsevier B. V.
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