期刊
AMERICAN JOURNAL OF CHINESE MEDICINE
卷 43, 期 3, 页码 559-579出版社
WORLD SCIENTIFIC PUBL CO PTE LTD
DOI: 10.1142/S0192415X15500354
关键词
Artocarpus communis; Hepatocellular Carcinoma; Autophagy; Apoptosis; Antihepatoma Activity; Chemotherapy Agent
资金
- Chi-Mei Medical Center
- Kaohsiung Medical University Research Foundation [102CM-KMU06]
For centuries, natural plant extracts have played an important role in traditional medicine for curing and preventing diseases. Studies have revealed that Artocarpus communis possess various bioactivities, such as anti-inflammation, anti-oxidant, and anticancer activities. Artocarpus communis offers economic value as a source of edible fruit, yields timber, and is widely used in folk medicines. However, little is known about its molecular mechanisms of anticancer activity. Here, we demonstrate the antiproliferative activity of Artocarpus communis methanol extract (AM) and its dichloromethane fraction (AD) in two human hepatocellular carcinoma (HCC) cell lines, HepG2 and PLC/PRF/5. Colony assay showed the long-term inhibitory effect of both extracts on cell growth. DNA laddering and immunoblotting analyses revealed that both extracts did not induce apoptosis in the hepatoma cell lines. AM and AD-treated cells demonstrated different cell cycle distribution compared to UV-treated cells, which presented apoptotic cell death with high sub-G1 ratio. Instead, acridine orange staining revealed that AM and AD triggered autophagosome accumulation. Immunoblotting showed a significant expression of autophagy-related proteins, which indicated the autophagic cell death (ACD) of hepatoma cell lines. This study therefore demonstrates that Artocarpus communis AM and its dichloromethane fraction can induce ACD in HCC cells and elucidates the potential of Artocarpus communis extracts for development as anti tumor therapeutic agents that utilize autophagy as mechanism in mediating cancer cell death.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据