期刊
NATURE MICROBIOLOGY
卷 3, 期 1, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41564-017-0043-0
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资金
- Paul G. Allen Frontiers Group
- Canadian Institutes of Health Research, National Cancer Institute [5T32CA009216-34]
- US National Institutes of Health National Human Genome Research Institute [RM1 HG008525]
- Wyss Institute for Biologically Inspired Engineering
Candida albicans is the leading cause of fungal infections; yet, complex genetic interaction analysis remains cumbersome in this diploid pathogen. Here, we developed a CRISPR-Cas9-based 'gene drive array' platform to facilitate efficient genetic analysis in C. albicans. In our system, a modified DNA donor molecule acts as a selfish genetic element, replaces the targeted site and propagates to replace additional wild-type loci. Using mating-competent C. albicans haploids, each carrying a different gene drive disabling a gene of interest, we are able to create diploid strains that are homozygous double-deletion mutants. We generate double-gene deletion libraries to demonstrate this technology, targeting antifungal efflux and biofilm adhesion factors. We screen these libraries to identify virulence regulators and determine how genetic networks shift under diverse conditions. This platform transforms our ability to perform genetic interaction analysis in C. albicans and is readily extended to other fungal pathogens.
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