期刊
ACS SENSORS
卷 2, 期 1, 页码 57-60出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssensors.6b00679
关键词
glycan biosensor; surface plasmon resonance; BSA-sugar conjugate; mannose; concanavalin A
资金
- Program for New Century Excellent Talents in University [NCET-12-1064]
- Natural Science Foundation of China [21402140]
Controlled immobilization of sugar probes is of key importance for the development of glycan biosensors. To this end, a series of BSA-sugar conjugates with different numbers of mannose units are prepared via the squaric acid mediated coupling reaction. The conjugates can absorb directly on gold substrate without any derivation reactions, thus providing a simple and effective method for the construction of SPR-based glycan biosensors. SPR measurements show that the BSA-mannose conjugate with 11 mannoses exhibit the highest affinity to the lectin concanavalin A with a limit of detection of ca. 1.8 nM. Regeneration and specificity of the obtained glycan biosensors are also investigated.
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