期刊
ACS SENSORS
卷 2, 期 11, 页码 1644-1652出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssensors.7b00486
关键词
electrochemical biosensor; IFN-gamma aptamer; nanowire electrode; aptasensor; tuberculosis detection
资金
- National Science Foundation [CHE-1403561]
- Ministry of Education and Science of the Republic of Kazakhstan [4132/GF4]
- National Natural Science Foundation of China [21605083]
- Natural Science Foundation of Jiangsu Province [BK20160644]
- Direct For Mathematical & Physical Scien [1403561] Funding Source: National Science Foundation
- Division Of Chemistry [1403561] Funding Source: National Science Foundation
Cytokines are small proteins secreted by immune cells in response to pathogens/infections; therefore, these proteins can be used in diagnosing infectious diseases. For example, release of a cytokine interferon (IFN)-gamma from T-cells is used for blood-based diagnosis of tuberculosis (TB). Our lab has previously developed an atpamer-based electrochemical biosensor for rapid and sensitive detection of IFN-gamma. In this study, we explored the use of silicon nanowires (NWs) as a way to create nanostructured electrodes with enhanced sensitivity for IFN-gamma. Si NWs were covered with gold and were further functionalized with thiolated aptamers specific for IFN-gamma. Aptamer molecules were designed to form a hairpin and in addition to terminal thiol groups contained redox reporter molecules methylene blue. Binding of analyte to aptamer-modified NWs (termed here nanowire aptasensors) inhibited electron transfer from redox reporters to the electrode and caused electrochemical redox signal to decrease. In a series of experiments we demonstrate that NW aptasensors responded 3x faster and were 2x more sensitive to IFN-gamma compared to standard flat electrodes. Most significantly, NW aptasensors allowed detection of IFN-gamma from as few as 150 T-cells/mL while ELISA did not pick up signal from the same number of cells. One of the challenges faced by ELISA-based TB diagnostics is poor performance in patients whose T-cell numbers are low, typically HIV patients. Therefore, NW aptasensors developed here may be used in the future for more sensitive monitoring of IFN-gamma responses in patients coinfected with HIV/TB.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据