期刊
PROTEOMES
卷 4, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/proteomes4030020
关键词
ECM; proteoforms; phosphoproteome; potato; 2-DE; protein interaction network
资金
- National Institute of Plant Genome Research, New Delhi, India
- Department of Biotechnology, Govt. of India [BT/HRD/35/01/05/2013]
- DBT-TWAS
- Department of Biotechnology (DBT), Govt. of India
- Council of Scientific and Industrial research (CSIR), Govt. of India
The extracellular matrix (ECM) has a molecular machinery composed of diverse proteins and proteoforms that combine properties of tensile strength with extensibility exhibiting growth-regulatory functions and self- and non-self-recognition. The identification of ECM proteoforms is the prerequisite towards a comprehensive understanding of biological functions accomplished by the outermost layer of the cell. Regulatory mechanisms of protein functions rely on post-translational modifications, phosphorylation in particular, affecting enzymatic activity, interaction, localization and stability. To investigate the ECM proteoforms, we have isolated the cell wall proteome and phosphoproteome of a tuberous crop, potato (Solanum tuberosum). LC-MS/MS analysis led to the identification of 38 proteins and 35 phosphoproteins of known and unknown functions. The findings may provide a better understanding of biochemical machinery and the integrated protein and phosphoprotein network of ECM for future functional studies of different developmental pathways and guidance cues in mechanosensing and integrity signaling.
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