期刊
3 BIOTECH
卷 7, 期 -, 页码 -出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s13205-017-0890-7
关键词
Direct PCR; Wide range amplification; DNA extraction; Molecular screening and genotyping; Biomedical diagnosis; Molecular detection
资金
- Tunisian Ministry of Higher Education and Scientific Research
Taking into account the limits of current genotyping methodologies, we have established a versatile direct PCR method on intact microtissue samples without prior DNA isolation. A simple and standard protocol was developed and validated on a wide range of living organisms including bacterial and fungal strains, plant species and human samples. This allows reliable amplification of target genomic DNA fragment directly from source material using minimal amount of tissue which makes DNA purification irrelevant for a number of biological applications. The direct PCR technique established here represents an excellent alternative to traditional amplification methods used for real-time detection. Since this approach was efficiently and universally applied for high-throughput molecular screening, its implementation will offer new insights for several investigations in human health, biomedical diagnosis, plant biotechnology, as well as in applied environmental and food microbiology.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据