期刊
III INTERNATIONAL SYMPOSIUM ON CITRUS BIOTECHNOLOGY
卷 1135, 期 -, 页码 109-115出版社
INT SOC HORTICULTURAL SCIENCE
DOI: 10.17660/ActaHortic.2016.1135.13
关键词
calcium ion; actin filament; pollen culture; self-incompatibility; punctate foci
资金
- Grants-in-Aid for Scientific Research [16K07599] Funding Source: KAKEN
Self-incompatibility (SI) in Citrus strongly prevents self-pollination and promotes outcrossing. Even though it has been confirmed that the S-allele is a key gene in controlling the recognition between pollen and pistil in Rosaceae, Solanaceae and Papaveraceae, this has not been cloned in Citrus. A citrus mature pollen culture system was established in our lab in order to culture 'Banpeiyu' and 'Hyuganatsu' pollen in vitro. Stylar crude protein extracts were used to treat citrus pollen in order to induce the SI-like response in the pollen tube. We observed the alteration of the pollen tube shape, including tip twisting and content leakage. Programmed cell death (PCD), as a normal cellular process that eliminates unwanted cells, was reported in previous studies of incompatible pollen tubes, together with an increase in hydrogen peroxide (H2O2), depolymerization of actin skeleton, the collapse of mitochondria, and leakage of cytochrome c. In our study, we investigated the cytosolic calcium ion concentration ([Ca2+](cyt)) changes and identified alterations in the actin cytoskeleton in the pollen tube after exposure to the incompatible treatment. The [Ca2+](cyt) content increased after incompatible treatment, but there was no obvious difference between the compatible-treatment group and the control. Punctate foci of actin filaments were found in the incompatible-treated pollen tubes; the rates of punctate foci were significantly higher than in the compatible-treated pollen tubes in both 'Banpeiyu' and 'Hyuganatsu'. The results suggested that these alterations in the pollen tubes were directly induced by the SI-like response, and might cause pollen tube PCD.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据