期刊
ACS BIOMATERIALS SCIENCE & ENGINEERING
卷 3, 期 12, 页码 3563-3575出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsbiomaterials.7b00681
关键词
RT-PCR; human fibroblast-keratinocyte coculture; porous scaffold; rat wound model
资金
- Council of Scientific & Industrial Research (CSIR)
- Indian Institute of Technology, Kharagpur
- DBT, Govt. of India (CST) [BT/PR7818/MED/32/279/2013]
Electrospun nanofibrous scaffold has long been studied as skin substitutes for their structural resemblance to the dermal extracellular matrix. However, packed fibrous architecture with small pore size restricts cellular infiltration into nanofibrous mat. In this article, we report highly porous, nano-/microfibrous 3D structure using polycaprolactone-chitosan emulsion and its application in skin regeneration. Under the influence of electric field, the emulsion containing encapsulated charged chitosan droplets enhances charge of the spinning solution and residual charge in the core of the deposited fiber, thereby creating core-shell, cotton-like fluffy structure with average pore size 62 mu m, fiber diameter similar to 1.62 mu m, contact angle of 72 degrees and 80% water uptake capacity of the scaffold. Further, differential stirring period of the specific emulsion developed compact nanofibrous membrane with nanometer ranged pore size emphasizing the role played by emulsion droplet size and the charge carried thereafter. Presence of nanofibers with high-interconnected porosity promoted efficient cellular infiltration and proliferation from initial days of cell seeding. The scaffold supported extracellular matrix protein expression and stratified epithelialization in vitro. Effective integration and attachment of scaffold with margins of a full-thickness excision wound created in a rat model with accelerated healing within 3 weeks proved the efficiency of the scaffold as skin substitute. Additionally, gradual and prolong release of acidic chitosan from the core section benefitted wound healing by lowering the pH of wound environment. Simple technique with inexpensive raw materials endorsed the scaffold as a promising off-the-shelf matrix for skin tissue regeneration.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据