期刊
FRONTIERS IN CHEMISTRY
卷 5, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fchem.2017.00102
关键词
extracellular vesicles; exosomes; proteomics; post-translational modification; mass spectrometry; bottom-up; top-down; crosslinking
资金
- CNPQ [441878/2014-8, 202077/2015-2]
- FAPESP [2014/06863-3]
- Italian Minister of Health [GR11.172.1]
- Ricerca Finalizzata fellowship [GR11.172.1]
- PBIC
Increasing attention has been given to secreted extracellular vesicles (EVs) in the past decades, especially in the portrayal of their molecular cargo and role as messengers in both homeostasis and pathophysiological conditions. This review presents the state-of-the-art proteomic technologies to identify and quantify EVs proteins along with their PTMs, interacting partners and structural details. The rapid growth of mass spectrometry-based analytical strategies for protein sequencing, PTMs and structural characterization has improved the level of molecular details that can be achieved from limited amount of EVs isolated from different biological sources. Here we will provide a perspective view on the achievements and challenges on EVs proteome characterization using mass spectrometry. A detailed bioinformatics approach will help us to picture the molecular fingerprint of EVs and understand better their pathophysiological function.
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