期刊
PHOTOACOUSTICS
卷 4, 期 2, 页码 43-54出版社
ELSEVIER GMBH
DOI: 10.1016/j.pacs.2016.04.001
关键词
Hepatocellular carcinoma; Epidermal growth factor receptor (EGFR); Photoacoustic; Imaging; Peptide
类别
资金
- National Institutes of Health [U54CA163059, U01CA189291, R01 CA142750, R01 CA200007, R01 EB020644]
EGFR is a promising cell surface target for in vivo imaging that is highly overexpressed in hepatocellular carcinoma (HCC), a common cancer worldwide. Peptides penetrate easily into tumors for deep imaging, and clear rapidly from the circulation to minimize background. We aim to demonstrate use of an EGFR specific peptide to detect HCC xenograft tumors in mice with photoacoustic imaging. Nude mice implanted with human HCC cells that overexpress EGFR were injected intravenously with Cy5.5-labeled EGFR and scrambled control peptides respectively. Photoacoustic images collected from 0 to 24 h. Photoacoustic signal peaked in tumors at 3 h post-injection. Images from 0 to 1.8 cm beneath the skin revealed increased target-to-background (T/B) ratio from tumors. The T/B ratio was significantly greater for the EGFR versus control peptide. Clearance of signal was observed by similar to 24 h. EGFR overexpression was validated with immunofluorescence and immunohistochemistry. A peptide specific for EGFR delivered systemically can detect HCC xenograft tumors in vivo with photoacoustic imaging. (C) 2016 Published by Elsevier GmbH.
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