期刊
SCIENTIFIC DATA
卷 4, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/sdata.2017.93
关键词
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资金
- CNRS (in particular Groupement de Recherche) [GDR3280]
- European Molecular Biology Laboratory (EMBL)
- Genoscope/CEA
- French Government 'Investissements d'Avenir' programme OCEANOMICS [ANR-11-BTBR-0008]
- French Government 'Investissements d'Avenir' programme FRANCE GENOMIQUE [ANR-10-INBS-09-08]
- Agence Nationale de la Recherche
- European Union FP7 (MicroB3) [287589]
- U.S. National Science Foundation [DEB-1031049, OCE-0623288, OCE-821374, OCE-1019242, OCE-1335810]
- Spanish Ministry of Science and Innovation grant [CGL2011-26848/BOS MicroOcean PANGENOMICS]
- Japan Society for the Promotion of Science (JSPS)/KAKENHI [26430184, 16H06429, 16K21723, 16H06437]
- Veolia Environment Foundation
- Region Bretagne
- Lorient Agglomeration
- World Courier
- Illumina
- Electricite de France (EDF) Foundation
- Fondation pour la recherche sur la biodiversite (FRB)
- Foundation Prince Albert II de Monaco
- Tara Foundation
- Grants-in-Aid for Scientific Research [16H06429, 16H06437] Funding Source: KAKEN
- Directorate For Geosciences
- Division Of Ocean Sciences [1335810] Funding Source: National Science Foundation
- Division Of Environmental Biology
- Direct For Biological Sciences [1441717] Funding Source: National Science Foundation
- Biotechnology and Biological Sciences Research Council [BB/I02612X/1] Funding Source: researchfish
- BBSRC [BB/I02612X/1] Funding Source: UKRI
A unique collection of oceanic samples was gathered by the Tara Oceans expeditions (2009-2013), targeting plankton organisms ranging from viruses to metazoans, and providing rich environmental context measurements. Thanks to recent advances in the field of genomics, extensive sequencing has been performed for a deep genomic analysis of this huge collection of samples. A strategy based on different approaches, such as metabarcoding, metagenomics, single-cell genomics and metatranscriptomics, has been chosen for analysis of size-fractionated plankton communities. Here, we provide detailed procedures applied for genomic data generation, from nucleic acids extraction to sequence production, and we describe registries of genomics datasets available at the European Nucleotide Archive (ENA, www. ebi. ac. uk/ena). The association of these metadata to the experimental procedures applied for their generation will help the scientific community to access these data and facilitate their analysis. This paper complements other efforts to provide a full description of experiments and open science resources generated from the Tara Oceans project, further extending their value for the study of the world's planktonic ecosystems. [GRAPHICS]
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