期刊
CELL SYSTEMS
卷 4, 期 5, 页码 505-+出版社
CELL PRESS
DOI: 10.1016/j.cels.2017.04.008
关键词
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资金
- Australian Research Council - LanzaTech [ARC LP140100213]
- QLD node of the National Biologics Facility
- initiative of the Australian Government being conducted as part of the NCRIS National Research Infrastructure for Australia
- DCSEI at the University of Queensland
Acetogens are promising cell factories for producing fuels and chemicals from waste feedstocks via gas fermentation, but quantitative characterization of carbon, energy, and redox metabolism is required to guide their rational metabolic engineering. Here, we explore acetogen gas fermentation using physiological, metabolomics, and transcriptomics data for Clostridium autoethanogenum steady-state chemostat cultures grown on syngas at various gas-liquid mass transfer rates. We observe that C. autoethanogenum shifts from acetate to ethanol production to maintain ATP homeostasis at higher biomass concentrations but reaches a limit at a molar acetate/ethanol ratio of similar to 1. This regulatory mechanism eventually leads to depletion of the intracellular acetyl-CoA pool and collapse of metabolism. We accurately predict growth phenotypes using a genome-scale metabolic model. Modeling revealed that themethylene-THF reductase reaction was ferredoxin reducing. This work provides a reference dataset to advance the understanding and engineering of arguably the first carbon fixation pathway on Earth.
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