期刊
CELL SYSTEMS
卷 4, 期 4, 页码 393-+出版社
CELL PRESS
DOI: 10.1016/j.cels.2017.03.001
关键词
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资金
- Marie Curie Career Integration Grant (CIG) [303507]
- Austrian Science Fund (FWF) [P 27201-B22]
- HFSP program [RGP0042/2013]
- Austrian Science Fund (FWF) [P27201] Funding Source: Austrian Science Fund (FWF)
Antibiotics elicit drastic changes in microbial gene expression, including the induction of stress response genes. While certain stress responses are known to cross-protect bacteria fromother stressors, it is unclear whether cellular responses to antibiotics have a similar protective role. By measuring the genome-wide transcriptional response dynamics of Escherichia coli to four antibiotics, we found that trimethoprim induces a rapid acid stress response that protects bacteria from subsequent exposure to acid. Combining microfluidics with time-lapse imaging to monitor survival and acid stress response in single cells revealed that the noisy expression of the acid resistance operon gadBC correlates with single-cell survival. Cells with highergadBCexpression following trimethoprim maintain higher intracellular pH and survive the acid stress longer. The seemingly randomsingle-cell survival under acid stress can therefore be predicted from gadBC expression and rationalized in terms of GadB/C molecular function. Overall, we provide a roadmap for identifying the molecular mechanisms of single-cell cross-protection between antibiotics and other stressors.
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