期刊
PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS
卷 23, 期 3, 页码 597-604出版社
SPRINGER
DOI: 10.1007/s12298-017-0456-5
关键词
C. annuum; CanPI7; Transgenic tomato; Proteinase inhibitor; H. armigera
资金
- CSIR
- Science and Engineering Research Board, Department of Science & Technology, Government of India, New Delhi [SB/YS/LS-260/2013]
- CSIR under XII five year plan program [BSC0107]
Plant proteinase inhibitors (PIs) are plant defense proteins and considered as potential candidates for engineering plant resistances against herbivores. Capsicum annuum proteinase inhibitor (CanPI7) is a multi-domain potato type II inhibitor (Pin-II) containing four inhibitory repeat domains (IRD), which target major classes of digestive enzymes in the gut of Helicoverpa armigera larvae. Stable integration and expression of the transgene in T1 transgenic generation, were confirmed by established molecular techniques. Protein extract of transgenic tomato lines showed increased inhibitory activity against H. armigera gut proteinases, supporting those domains of CanPI7 protein to be effective and active. When T1 generation plants were analyzed, they exhibited antibiosis effect against first instar larvae of H. armigera. Further, larvae fed on transgenic tomato leaves showed delayed growth relative to larvae fed on control plants, but did not change mortality rates significantly. Thus, better crop protection can be achieved in transgenic tomato by overexpression of multi-domain proteinase inhibitor CanPI7 gene against H. armigera larvae.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据