期刊
BIOFABRICATION
卷 8, 期 4, 页码 -出版社
IOP PUBLISHING LTD
DOI: 10.1088/1758-5090/8/4/045001
关键词
additive manufacturing; hydrogels; 3D plotting; 3D printing; extrusion; growth factor delivery
资金
- German Federal Ministry for Economic Affairs and Energy (BMWi)
- German Federation of Industrial Research Associations (AiF) (ZIM project) [KF3359301AK4]
- Saxon State Ministry for Higher Education and Arts (SMWK) [4-7531.60/29/24]
- German Federal and State Governments (Institutional Strategy of TU Dresden)
Three-dimensional extrusion of two different biomaterials in a core/shell (c/s) fashion has gained much interest in the last couple of years as it allows for fabricating constructs with novel and interesting properties. Wenow demonstrate that combining high concentrated (16.7 wt%) alginate hydrogels as shell material with low concentrated, soft biopolymer hydrogels as core leads to mechanically stable and robust 3D scaffolds. Alginate, chitosan, gellan gum, gelatin and collagen hydrogels were utilized successfully as core materials-hydrogels which are too soft for 3D plotting of open-porous structures without an additional mechanical support. The respective c/s scaffolds were characterized concerning their morphology, mechanical properties and swelling behavior. It could be shown that core as well as shell part can be loaded with growth factors and that the release depends on core composition and shell thickness. Neither the plotting process nor the crosslinking with 1M CaCl2 denatured the proteins. When core and shell were loaded with different growth factors (VEGF and BMP-2, respectively) a dual release was achieved. Finally, live human endothelial cells were integrated in the core material, demonstrating that this new strategy can be used for bioprinting purposes as well.
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