期刊
NATURE PLANTS
卷 2, 期 12, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NPLANTS.2016.183
关键词
-
资金
- National Key R & D Program for Crop Breeding [2016YFD0100307]
- National Science Fund for Distinguished Young Scholars [31225025]
- National Program on Key Basic Research Projects in China (the 973 Program) [2012CB113900]
- leading talents of Guangdong province Program [00201515]
- National Natural Science Foundation of China [31322047, 31401886, 31101550, 31672171]
- Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences (CAAS-ASTIP-IVFCAAS)
- Chinese Ministry of Finance [1251610601001]
- China Postdoctoral Science Foundation [2014M550902]
- Discovery Grant from National Science and Engineering Research Council of Canada (NSERC)
- Canada Research Chair program
- UK Biotechnological and Biological Sciences Research Council Institute Strategic Programme Grant 'Understanding and Exploiting Plant and Microbial Metabolism' [BB/J004561/1]
- John Innes Foundation
- Genomes to Natural Products National Institutes of Health Programme [U01GM110699]
- Shenzhen Municipal Government
- Dapeng District Government
- BBSRC [BB/L014130/1, BBS/E/J/000CA533, BBS/E/J/000CA527] Funding Source: UKRI
- EPSRC [EP/K034359/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BBS/E/J/000CA533, BB/L014130/1, BBS/E/J/00000614, BBS/E/J/000CA527] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/K034359/1] Funding Source: researchfish
Differentiation of secondary metabolite profiles in closely related plant species provides clues for unravelling biosynthetic pathways and regulatory circuits, an area that is still underinvestigated. Cucurbitacins, a group of bitter and highly oxygenated tetracyclic triterpenes, are mainly produced by the plant family Cucurbitaceae. These compounds have similar structures, but differ in their antitumour activities and ecophysiological roles. By comparative analyses of the genomes of cucumber, melon and watermelon, we uncovered conserved syntenic loci encoding metabolic genes for distinct cucurbitacins. Characterization of the cytochrome P450s (CYPs) identified from these loci enabled us to unveil a novel multi-oxidation CYP for the tailoring of the cucurbitacin core skeleton as well as two other CYPs responsible for the key structural variations among cucurbitacins C, B and E. We also discovered a syntenic gene cluster of transcription factors that regulates the tissue-specific biosynthesis of cucurbitacins and may confer the loss of bitterness phenotypes associated with convergent domestication of wild cucurbits. This study illustrates the potential to exploit comparative genomics to identify enzymes and transcription factors that control the biosynthesis of structurally related yet unique natural products.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据