期刊
CLINICAL MASS SPECTROMETRY
卷 2, 期 -, 页码 1-10出版社
ELSEVIER
DOI: 10.1016/j.clinms.2016.11.002
关键词
Ion mobility spectrometry; Mass spectrometry; Metabolomics; Exposomics
资金
- National Institute of Environmental Health Sciences of the NIH [R01 ES022190]
- National Institute of General Medical Sciences [P41 GM103493]
- Laboratory Directed Research and Development Program
- Microbes in Transition (MinT) Initiative at Pacific Northwest National Laboratory
- U.S. Department of Energy Office of Biological and Environmental Research Genome Sciences Program
- National Institute of Allergy and Infectious Diseases [U19 AI106772]
- DOE [DE-AC05-76RL0 1830]
Characterization of endogenous metabolites and xenobiotics is essential to deconvoluting the genetic and environmental causes of disease. However, surveillance of chemical exposure and disease-related changes in large cohorts requires an analytical platform that offers rapid measurement, high sensitivity, efficient separation, broad dynamic range, and application to an expansive chemical space. Here, we present a novel platform for small molecule analyses that addresses these requirements by combining solid-phase extraction with ion mobility spectrometry and mass spectrometry (SPE-IMS-MS). This platform is capable of performing both targeted and global measurements of endogenous metabolites and xenobiotics in human biofluids with high reproducibility (CV <= 3%), sensitivity (LODs in the pM range in biofluids) and throughput (10-s sample-to-sample duty cycle). We report application of this platform to the analysis of human urine from patients with and without type 1 diabetes, where we observed statistically significant variations in the concentration of disaccharides and previously unreported chemical isomers. This SPE-IMS-MS platform overcomes many of the current challenges of large-scale metabolomic and exposomic analyses and offers a viable option for population and patient cohort screening in an effort to gain insights into disease processes and human environmental chemical exposure. (C) 2017 The Association for Mass Spectrometry: Applications to the Clinical Lab (MSACL). Published by Elsevier B.V.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据