期刊
MAMMAL RESEARCH
卷 62, 期 4, 页码 405-411出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s13364-017-0328-y
关键词
mtDNA; COIIgene; Molecular ecology; Canid; Biodiversity monitoring; Uruguay
类别
资金
- Programa de Desarrollo de las Ciencias Basicas (PEDECIBA)
- Agencia Nacional de Investigacion e Innovacion (ANII) [FCE_2_20011_1_5700]
Strategies to evaluate and monitor elusive mammal species require the development of genetic techniques and their application to unambiguous biological material for ecological and genetic studies. In order to assess cytochrome c oxidase subunit II gene inter- and intraspecific variations, we compared sequences from different Neotropical canids and domestic dogs. We developed a primer pair to amplify a 154-bp fragment of this gene and a species-specific multiplex TaqMan (TM) assay for accurate identification of two native fox species occurring in sympatry in South America, the crabeating fox (Cerdocyon thous) and the pampas fox (Lycalopex gymnocercus). The assays can also distinguish domestic dogs (Canis lupus familiaris) from both wild foxes. The use of different fluorescent reporter dyes for species identification in a multiplex probe PCR-RT assay reduces labor and costs. The methodology presented in this study demonstrates an efficient approach to enable high-performance analysis and represents a reliable cost-effective tool for molecular ecology research to monitor the wild canid populations by noninvasive genetic sampling. This standardized assay will allow large-scale high-throughput analyses in a routine and reliable way.
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