期刊
JOURNAL OF APPLIED CRYSTALLOGRAPHY
卷 50, 期 -, 页码 1773-1781出版社
INT UNION CRYSTALLOGRAPHY
DOI: 10.1107/S1600576717014479
关键词
serial synchrotron crystallography; SSX; picosecond infrared lasers; PIRL; fixed targets; sample delivery
资金
- Max Planck Institute
- excellence cluster 'The Hamburg Centre for Ultrafast Imaging - Structure, Dynamics and Control of Matter at the Atomic Scale' of the Deutsche Forschungsgemeinschaft
- National Science and Engineering Council of Canada [RGPIN-2015-04877]
- People Programme (Marie Curie Actions) of the European Union's Seventh Framework Programme (FP7) under REA grant [623994]
- Canada Research Chairs program
- Burroughs Wellcome Fund
In order to utilize the high repetition rates now available at X-ray free-electron laser sources for serial crystallography, methods must be developed to softly deliver large numbers of individual microcrystals at high repetition rates and high speeds. Picosecond infrared laser (PIRL) pulses, operating under desorption by impulsive vibrational excitation (DIVE) conditions, selectively excite the OH vibrational stretch of water to directly propel the excited volume at high speed with minimized heating effects, nucleation formation or cavitationinduced shock waves, leaving the analytes intact and undamaged. The soft nature and laser-based sampling flexibility provided by the technique make the PIRL system an interesting crystal delivery approach for serial crystallography. This paper demonstrates that protein crystals extracted directly from aqueous buffer solution via PIRL-DIVE ablation retain their diffractive properties and can be usefully exploited for structure determination at synchrotron sources. The remaining steps to implement the technology for high-speed serial femtosecond crystallography, such as single-crystal localization, high-speed sampling and synchronization, are described. This proof-of-principle experiment demonstrates the viability of a new laser-based high-speed crystal delivery system without the need for liquid-jet injectors or fixed-target mounting solutions.
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