期刊
IUCRJ
卷 4, 期 -, 页码 795-811出版社
INT UNION CRYSTALLOGRAPHY
DOI: 10.1107/S2052252517014324
关键词
serial crystallography; coherent X-ray diffractive imaging (CXDI); single particles; molecular orientation determination; crystalline fibrils; amyloid
资金
- New Zealand government
- Helmholtz Association (Germany)
- US Department of Energy through Lawrence Livermore National Laboratory [DE-AC52-07NA27344]
- NIH [1R01GM117342-01]
- FRISBI [ANR-10-INSB-05-02]
- GRAL within Grenoble Partnership for Structural Biology (PSB) [ANR-10-LABX-49-01]
- US Department of Energy, Office of Science, Office of Basic Energy Sciences [DE-AC02-76SF00515]
Serial diffraction data collected at the Linac Coherent Light Source from crystalline amyloid fibrils delivered in a liquid jet show that the fibrils are well oriented in the jet. At low fibril concentrations, diffraction patterns are recorded from single fibrils; these patterns are weak and contain only a few reflections. Methods are developed for determining the orientation of patterns in reciprocal space and merging them in three dimensions. This allows the individual structure amplitudes to be calculated, thus overcoming the limitations of orientation and cylindrical averaging in conventional fibre diffraction analysis. The advantages of this technique should allow structural studies of fibrous systems in biology that are inaccessible using existing techniques.
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