期刊
FEMS MICROBIOLOGY LETTERS
卷 363, 期 18, 页码 -出版社
OXFORD UNIV PRESS
DOI: 10.1093/femsle/fnw204
关键词
(1 -> 3)-beta-D-glucan; gastrointestinal leakage; Clostridium difficile; murine model
类别
资金
- Grant for Development of New Faculty Staff
- Grant for International Research Integration
- Chula Research Scholar
- Ratchadaphiseksomphot Endowment Fund [CU-56-533-HR]
- National Research University Project, Office of Higher Education Commission, Chulalongkorn University [NRU59-032-HR]
- Associates of Cape Cod, Inc.
Gastrointestinal (GI) leakage in Clostridium difficile-associated diarrhea (CDAD) is well known but is not routinely assessed in clinical practice. Serum (1 -> 3)-beta-D-glucan (BG), a fungal cell wall component used as a biomarker for invasive fungal disease, was tested in a CDAD mouse model with and without probiotics. Higher serum fluorescein isothiocyanate-dextran (FITC-dextran) and spontaneous gram-negative bacteremia, GI leakage indicators, were frequently found in CDAD mice, which died compared with those which survived. BG, serum macrophage inflammatory protein-2 and FITC-dextran but not quantitative blood bacterial count differentiated the clinical severity. Interestingly, a specific dose of Lactobacillus rhamnosus L34 attenuated CDAD and decreased serum BG and FITC-dextran, but not other parameters. BG also showed a higher area under the receiver operating characteristic curve for 7-day mortality than FITC-dextran. Fifty-five percent of CDAD mice with BG >= 60 pg/ml (the human negative cut-off value for invasive fungal disease) at 1 day after C. difficile gavage died within 7 days. In conclusion, serum BG was elevated in mice with severe CDAD, an established model of GI leakage with a strong association with mortality rate. BG monitoring in patients with CDAD is of interest as both a potential prognostic tool and a therapeutic efficacy indicator.
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