期刊
INFLAMMOPHARMACOLOGY
卷 25, 期 5, 页码 523-532出版社
SPRINGER BASEL AG
DOI: 10.1007/s10787-017-0385-5
关键词
Paeonol; Inflammation; Human fibroblast-like synoviocytes; NF-kappa B signalling pathway; Collagen-induced arthritis
资金
- National Natural Science Foundation of China [81601034]
- Anhui Natural Science Foundation [1608085QH185]
- Key Natural Science Foundation of Anhui Educational Committee [KJ2015A220]
- Key Project Fund for Outstanding Young Talents in Anhui Higher Education Institutions [gxyqZD2016348]
- Postdoctoral Foundation of Jiangsu Province [1601173B]
- Suzhou Regional Development Collaborative Innovation Center [2016szxt03, 2015SZXTZXKFYB03]
- Open Project Program of the Institute of Pharmaceutical Biotechnology [2015YKF06]
Various investigations have demonstrated that human fibroblast-like synoviocytes rheumatoid arthritis (HFLS-RA) take part in the chronic inflammatory responses and RA progression. Inhibition of synovium activation and inflammatory processes may represent a therapeutic target to alleviate RA. Paeonol, a major natural product, has many biological and pharmacological activities. However, its protective effects against RA considering HFLS-RA have not been explored. In this study, anti-inflammatory effects of paeonol were detected in interleukin-1 beta (IL-1 beta)-treated HFLS-RA. Our results demonstrated that paeonol had no effect on cell survival and IL-1 beta-induced proliferation in HFLS-RA. Pretreatment with paeonol significantly suppressed the production of pro-inflammatory TNF-alpha, IL-6 and IL-1 beta, and the expressions of matrix metalloproteinase-1/-3 in vitro and in vivo. Mice treated with paeonol (10 mg/kg) remarkablely attenuated arthritic symptoms based on clinical arthritis scores and histopathology in collagen-induced arthritis mice. Furthermore, the TLR4 expression and NF-kappa B p65 activation were inhibited by paeonol in vitro and in vivo. Our findings illustrated that paeonol had significantly suppressed inflammation effects in synovial tissues and RA progression. The potential mechanism might be based on the attenuation TLR4-NF-kappa B activation. These collective results indicated that paeonol might be a promising therapeutic agent for alleviating RA progress through inhibiting inflammations and NF-kappa B signalling pathway.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据