期刊
FRONTIERS IN IMMUNOLOGY
卷 8, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fimmu.2017.01390
关键词
CCL3; natural killer cells; CD103(+) dendritic cells; lymphocytes; interferon-gamma; lymph node
类别
资金
- National Cancer Institute [R01CA154656, R21CA181875, NCI R01CA154656-S1, F31CA192874]
- St. Baldrick's Foundation
- Alex's Lemonade Stand Foundation
- Hyundai Hope-on-Wheels Program
- Pediatric Cancer Research Foundation
- Steven G. AYA Cancer Research Fund
- Samuel Szabo Foundation
- Keira Kilbane Cancer Discovery Fund
- Errol's Cancer Discovery Fund
- Theresia G. & Stuart F. Kline Family Foundation
Lymph node (LN) plays a critical role in tumor cell survival outside of the primary tumor sites and dictates overall clinical response in many tumor types (1, 2). Previously, we and others have demonstrated that CCL3 plays an essential role in orchestrating T cell-antigen-presenting cell (APC) encounters in the draining LN following vaccination, and such interactions enhance the magnitude of the memory T cell pool (3-5). In the current study, we investigate the cellular responses in the tumor-draining lymph nodes (TDLNs) of a CCL3-secreting CT26 colon tumor (L3TU) as compared to wild-type tumor (WTTU) during the priming phase of an antitumor response (<= 10 days). In comparison to WTTU, inoculation of L3TU resulted in suppressed tumor growth, a phenomenon that is accompanied by altered in vivo inflammatory responses on several fronts. Autologous tumor-derived CCL3 (aCCL3) secretion by L3TU bolstered the recruitment of T-and B-lymphocytes, tissue-migratory CD103(+) dendritic cells (DCs), and CD49b(+) natural killer (NK) cells, resulting in significant increases in the differentiation and activation of multiple Interferon-gamma (IFN gamma)-producing leukocytes in the TDLN. During this early phase of immune priming, NK cells constitute the major producers of IFN gamma in the TDLN. CCL3 also enhances CD8+ T cell proliferation and differentiation by augmenting DC capacity to drive T cell activation in the TDLN. Our results revealed that CCL3-dependent IFN. production and CCL3-induced DC maturation drive the priming of effective antitumor immunity in the TDLN.
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