期刊
FRONTIERS IN IMMUNOLOGY
卷 8, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fimmu.2017.00849
关键词
A(2B) adenosine receptor; cAMP; extracellular signal-regulated protein kinases; CD40; IL-12p70; dendritic cell; Leishmania amazonensis
类别
资金
- FAPEMIG
- CNPq
- CAPES
- UFOP
- Rede de Pesquisa em Doencas Infecciosas Humanas e Animais no Estado de Minas Gerais/FAPEMIG
- Rede Mineira de Bioterismo/FAPEMIG
Differently from others Leishmania species, infection by the protozoan parasite L. amazonensis is associated with a lack of antigen-specific T-cell responses. Dendritic cells (DC) are essential for the innate immune response and for directing the differentiation of T-helper lymphocytes. Previously, we showed that L. amazonensis infection impairs DC activation through the activation of adenosine A(2B) receptor, and here, we evaluated the intracellular events triggered by this receptor in infected cells. To this aim, bone marrow-derived DC from C57BL/6J mice were infected with metacyclic promastigotes of L. amazonensis. Our results show, for the first time, that L. amazonensis increases the production of cAMP and the phosphorylation of extracellular signal-regulatedprotein kinases 1/2 (ERK1/2) in infected DC by a mechanism dependent on the A(2B) receptor. Furthermore, L. amazonensis impairs CD40 expression and IL-12 production by DC, and the inhibition of adenylate cyclase, phosphoinositide 3-kinase (PI3K), and ERK1/2 prevent these effects. The increase of ERK1/2 phosphorylation and the inhibition of DC activation by L. amazonensis are independent of protein kinase A (PKA). In addition, C57BL/6J mice were inoculated in the ears with metacyclic promastigotes, in the presence of PSB1115, an A(2B) receptor antagonist. PSB1115 treatment increases the percentage of CD40(+) DC on ears and draining lymph nodes. Furthermore, this treatment reduces lesion size and tissue parasitism. Lymph node cells from treated mice produce higher levels of IFN-gamma than control mice, without altering the production of IL-10. In conclusion, we suggest a new pathway used by the parasite (A(2B) receptor -> cAMP -> PI3K -> ERK1/2) to suppress DC activation, which may contribute to the decrease of IFN-gamma production following by the deficiency in immune response characteristic of L. amazonensis infection.
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