期刊
AMB EXPRESS
卷 7, 期 -, 页码 -出版社
BIOMED CENTRAL LTD
DOI: 10.1186/s13568-017-0441-y
关键词
Monascus ruber; Water-soluble yellow pigments; Strong fluorescence; Gene regulation; Temperature-associated
资金
- National Natural Science Foundation of China [31271925]
- Special Project on the Integration of Industry, Education and Research of Guangdong Province, China [2013B090600015]
- Major Project on synergy innovation in enterprise-university-institute of Guangzhou, China [201604046011]
Monascus species can produce secondary metabolites that have a polyketide structure. In this study, four types of extracellular water-soluble yellow pigments (Y1-Y4) were generated by submerged fermentation with Monascus ruber CGMCC 10910, of which Y3 and Y4 had strong yellow fluorescence. The composition of the pigment mixtures was closely related to the fermentation temperature. The dominating pigments changed from Y1 to Y3 and Y4 when fermentation temperature increased from 30 to 35 degrees C. Increasing the temperature to 35 degrees C changed the metabolic pathways of the pigments, which inhibited the biosynthesis of Y1 and enhanced the biosynthesis of Y3 and Y4. Moreover, the yield of Y1 reduced insignificantly, while the yields of Y3 and Y4 increased by 98.21 and 79.31% respectively under two-stage temperature fermentation condition. The expression levels of the relative pigment biosynthetic genes, such as MpFasA2, MpFasB2, MpPKS5, mppR1, mppB, and mppE, were up-regulated at 35 degrees C. The two-stage temperature strategy is a potential method for producing water-soluble Monascus yellow pigments with strong yellow fluorescence.
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