期刊
ADVANCED SCIENCE
卷 4, 期 10, 页码 -出版社
WILEY
DOI: 10.1002/advs.201700034
关键词
anti-inflammation; cerium oxide nanoparticles; functional recovery; reactive oxygen species; spinal cord injury
资金
- National Research Foundation (NRF) [2009-0093829, 2015032163, 2015R1D1A1A02061196]
- Korea Healthcare Technology R&D Project, Republic of Korea [HI14C0522]
- National Research Foundation of Korea [2009-0093829, 2015R1D1A1A02061196] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Spinal cord injury (SCI) produces excess reactive oxygen species (ROS) that can exacerbate secondary injury and lead to permanent functional impairment. Hypothesizing that cerium oxide nanoparticles (CONPs) as an effective ROS scavenger may offset this damaging effect, it is first demonstrated in vitro that CONPs suppressed inducible nitric oxide synthase (iNOS) generation and enhanced cell viability of hydrogen peroxide (H2O2)-insulted cortical neurons. Next, CONPs are administered at various does (50-4000 mu g mL(-1)) to a contused spinal cord rat model and monitored the disease progression for up to eight weeks. At one day postinjury, the number of iNOS+ cells decreases in the treated groups compared with the control. At one week, the cavity size and inflammatory cells are substantially reduced, and the expression of proinflammatory and apoptotic molecules is downregulated with a concurrent upregulation of anti-inflammatory cytokine. By eight weeks, the treated groups show significantly improved locomotor functions compared with the control. This study shows for the first time that injection of optimal-dosed CONPs alone into contusion-injured spinal cord of rats can reduce ROS level, attenuate inflammation and apoptosis, and consequently help locomotor functional recovery, adding a promising and complementary strategy to the other treatments of acute SCI.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据