期刊
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
卷 73, 期 -, 页码 45-50出版社
INT UNION CRYSTALLOGRAPHY
DOI: 10.1107/S2053230X16020082
关键词
HAI-1; hepatocyte growth factor activator inhibitor; Drosophila; expression; purification; crystallization
资金
- Natural Science Foundation of China [31170707, 31370737, 31400637, 31570745, 31670739, U1405229]
Hepatocyte growth factor activator inhibitor 1 (HAI-1) is a multi-domain membrane-associated protease inhibitor that potently inhibits a variety of serine proteases such as hepatocyte growth factor activator and matriptase. Different truncates of HAI-1 show varying potencies for inhibition of target proteases, suggesting that the domain organization of HAI-1 plays a critical role in its function. Here, the soluble full-length extracellular part of HAI-1 (sHAI-1) was expressed using the Drosophila S2 insect-cell expression system. Diffraction-quality crystals of sHAI-1 were produced using ammonium sulfate as precipitant. The crystal diffracted to 3.8 angstrom resolution and belonged to space group P41212, with unit-cell parameters a = b = 95.42, c = 124.50 angstrom. The asymmetric unit contains one sHAI-1 molecule.
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