期刊
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
卷 73, 期 -, 页码 503-508出版社
INT UNION CRYSTALLOGRAPHY
DOI: 10.1107/S2059798317004181
关键词
cryo-EM; Electron Microscopy Data Bank; EMDB; resolution; direct electron detector; electron tomography
资金
- National Institutes of Health, National Institute of General Medical Sciences [R01 GM079429]
- Medical Research Council [MR/L007835]
- Biotechnology and Biological Sciences Research Council [BB/M018423/1]
- Wellcome Trust [088944, 104948]
- Seventh Framework Programme [284209]
- BBSRC [BB/G022577/1, BB/M018423/1] Funding Source: UKRI
- MRC [MR/L007835/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/M018423/1] Funding Source: researchfish
Recent technological advances, such as the introduction of the direct electron detector, have transformed the field of cryo-EM and the landscape of molecular and cellular structural biology. This study analyses these trends from the vantage point of the Electron Microscopy Data Bank (EMDB), the public archive for three-dimensional EM reconstructions. Over 1000 entries were released in 2016, representing almost a quarter of the total number of entries (4431). Structures at better than 6 angstrom resolution now represent one of the fastest-growing categories, while the share of annually released tomography-related structures is approaching 20%. The use of direct electron detectors is growing very rapidly: they were used for 70% of the structures released in 2016, in contrast to none before 2011. Microscopes from FEI have an overwhelming lead in terms of usage, and the use of the RELION software package continues to grow rapidly after having attained a leading position in the field. China is rapidly emerging as a major player in the field, supplementing the US, Germany and the UK as the big four. Similarly, Tsinghua University ranks only second to the MRC Laboratory for Molecular Biology in terms of involvement in publications associated with cryo-EM structures at better than 4 angstrom resolution. Overall, the numbers point to a rapid democratization of the field, with more countries and institutes becoming involved.
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