期刊
ACS INFECTIOUS DISEASES
卷 4, 期 1, 页码 46-52出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsinfecdis.7b00139
关键词
small intestine; leaky gut; bacterial translocation; NSAIDs; mitochondria; superoxide
资金
- NIH [CA098468, USDA 055336, CA207416, DK109559, T32DK007737]
- NCI [CA016086]
- NIEHS [P30ES010126-15A1]
- NCBT [2015-IDG-1007]
- University Cancer Research Fund (UCRF)
The intestinal epithelium provides a critical barrier that separates the gut microbiota from host tissues. Non steroidal anti-inflammatory drugs (NSAIDs) are efficacious analgesics and antipyretics and are among the most frequently used drugs worldwide. In addition to gastric damage, NSAIDs are toxic to the intestinal epithelium, causing erosions, perforations, and longitudinal ulcers in the gut. Here, we use a unique in vitro human primary small intestinal cell monolayer system to pinpoint the intestinal consequences of NSAID treatment. We found that physiologically relevant doses of the NSAID diclofenac (DCF) are cytotoxic because they uncouple mitochondrial oxidative phosphorylation and generate reactive oxygen species. We also find that DCF induces intestinal barrier permeability, facilitating the translocation of compounds from the luminal to the basolateral side of the intestinal epithelium. The results we outline here establish the utility of this novel platform, representative of the human small intestinal epithelium, to understand NSAID toxicity, which can be applied to study multiple aspects of gut barrier function including defense against infectious pathogens and host-microbiota interactions.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据