期刊
ACS INFECTIOUS DISEASES
卷 3, 期 8, 页码 575-584出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsinfecdis.7b00052
关键词
host pathogen interface; bacterial effector proteins; live cell imaging; split-GFP; Salmonella
资金
- NSF [MCB-0950411]
- NIH [T32 GM008732]
- [F31 GM106644]
The bacterial pathogen Salmonella uses sophisticated type III secretion systems (T3SS) to translocate and deliver bacterial effector proteins into host cells to establish infection. Monitoring these important virulence determinants in the context of live infections is a key step in defining the dynamic interface between the host and pathogen. Here, we Cij provide a modular labeling platform based on fluorescence complementation with split-GFP that permits facile tagging of new Salmonella effector proteins. We demonstrate enhancement of split-GFP complementation signals by manipulating the promoter or by multimerizing the fluorescent tag and visualize three effector proteins, SseF, SseG, and S1rP, that have never before been visualized over time during infection of live cells. Using this platform, we developed a methodology for visualizing effector proteins in primary macrophage cells for the first time and reveal distinct differences in the effector-defined intracellular niche between primary macrophage and commonly used HeLa and RAW cell lines.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据