期刊
ACS BIOMATERIALS SCIENCE & ENGINEERING
卷 3, 期 12, 页码 3687-3694出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsbiomaterials.7b00752
关键词
inkjet bioprinting; intersecting jets; reactive materials; neural stem cell; alginate; hydroxyapatite
资金
- US National Science Foundation [CMMI-1634755]
Although traditional three-dimensional bioprinting technology is suitable for many tissue engineering applications, various biomaterials and constructs call for bioprinting innovations. There is a need for the fabrication of complex structures from reactive biomaterials as well as heterogeneous structures with controlled material compositions. In particular, during reactive material printing, reactive solutions/suspensions that undergo changes in rheological properties or cytocompatibility are not printable using traditional bioprinting approaches that require all components of bioinks to be mixed before deposition. The objective of this study is to develop and implement an intersecting jets-based inkjet bioprinting approach, which enables voxel-resolution printing-then-mixing for the fabrication of biological structures using reactive materials as well as structures having a compositional gradient. Inkjetting is implemented herein as a versatile technique to simultaneously deposit droplets of disparate materials at controlled locations where active collision, mixing, and coalescence occur. For reactive material printing, neural stem cell (NSC) spheres are fabricated from reactive PuraMatrix hydrogel solution and physiological cell suspension, and cell-laden alginate structures are also printed in air directly from reactive sodium alginate and calcium chloride solutions. For heterogeneous structure printing, collagen sheets with a hydroxyapatite (HAP) content gradient are fabricated to demonstrate the unique online control of material composition throughout a structure. It is demonstrated that the proposed bioprinting approach is feasible for applications that utilize reactive materials or require heterogeneous compositions.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据